Fig 1: Identification of tissue-resident macrophages (RTMs). (A) UMAP plot of immune cells or non-immune cells. (B) UMAP plot of major immune cell types. (C) t-distributed stochastic neighbor embedding (t-SNE) plots displaying 10 macrophage clusters. (D) t-SNE plots showing the expression levels of representative genes. (E) Heatmap exhibiting the expression levels of representative genes across 10 macrophage clusters. RTM, tissue-resident macrophage; TAM, tumor-associated macrophages. (F) Violin plots showing the expression of RTM cluster-specific genes across five RTM clusters. RTM_1 cluster had no specific marker. (G,H) Immunofluorescence staining of CD68, MARCO (G), and FOLR2 (H) in BC tissues. Scale bar, 20 µm.
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